soluble antigens meaning in English
可溶性抗原
Examples
- The immunogenic potency of soluble antigens may be considerably increased .
它们的免疫原效能可以提高很多。 - Ts87 gene fragment was picked out by screening adult t . s cdna library using sera of rabbit raised against soluble antigen of t . s and using sera of rabbit infected artificially with t . s . the ts87 fragment was ligated to vector pcdnas . l , which contains a cmv promoter / enhancer
本研究采用通过免疫筛库得到的旋毛虫抗原基因片段ts87与载体pcdna3 . 1连接。该载体含有人类巨细胞病毒( cmv )的高效启动子和增强子,是一种外源基因在哺乳动物细胞内高效表达的理想载体。 - After the recombinant plasmid pcdna3 . 1 / ts87 was identified by digestion of hindlll and bamh i , it transformed into cos7 by lipofectamine . expression product was identified by immunohistochemical method , sds - page and western - blot . the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3 . 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas . l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t . s artificially and sera from rabbit immunized with soluble antigen of t . s and with protein expressed by ts87 gene and by a monoclonal antibody of t . s
通过细胞的免疫组化,细胞裂解物的sds - page电泳, westem - blot分析检测目的基因的表达情况。免疫组化结果显示:重组质粒转染的细胞质中有棕褐色颗粒,而空载体转染细胞及正常细胞无此现象;细胞裂解物sds - page电泳结果显示:只有重组质粒转染的细胞在约38kd处有明显的蛋白带,这与理论计算的ts87基因表达蛋白的分子量为38kd基本一致; western - blot分析结果显示:约38kd的蛋白带能够分别被旋毛虫感染兔血清,成虫虫体可溶性抗原免疫兔血清, ts87基因原核表达蛋白免疫兔血清( ts87血清)以及一株具保护性的旋毛虫单抗特异识别。